ABSTRACT
<p><b>OBJECTIVE</b>To investigate whether the protective effect of therapy with different combined neuroprotectant agents was better than that of single agent on focal cerebral ischemia.</p><p><b>METHODS</b>The right middle cerebral artery in the rats was occluded with suture occlusion technique. The rats were divided into five groups treated with FDP (50 mg/kg, n = 10), MK-801 (1 mg/kg, n = 10) and NAC (150 mg/kg, n = 10) singly, or in combination, respectively, by intraperitoneal infusion 30 minutes after vessel occlusion. The rats were weighed and assessed neurologically, based on a 5-point scale, six and 24 hours after focal cerebral ischemia. The expression of anti-apoptotic protein bcl-2 was observed with SDS-PAGE protein electrophoresis and Western blot technique.</p><p><b>RESULT</b>The optical density of bcl-2 increased more distinctly in the rats treated with combined neuroprotective agents than that with any single agent six and 24 hours after cerebral ischemia, with a statistically significant difference (P < 0.05).</p><p><b>CONCLUSIONS</b>Treatment with combined neuroprotectant agents could un-regulate the anti-apoptotic protein bcl-2 more distinctly than that with any single agents. Combined use of neuroprotectants might be more effective than that of single agent in protecting rats' brain from ischemia.</p>
Subject(s)
Animals , Male , Rats , Acetylcysteine , Actins , Brain Ischemia , Drug Therapy , Metabolism , Dizocilpine Maleate , Drug Therapy, Combination , Fructosediphosphates , Molecular Weight , Neuroprotective Agents , Proto-Oncogene Proteins c-bcl-2 , Rats, WistarABSTRACT
Objective To investigate whether cocktailtherapy combined with of neuroprotectants may have more advantages over single agents in treating focal cerebral ischemic cascade Methods Use suture occlusion technique, the right middle cerebral artery in rats was occluded Tirty minutes later,Fructose 1,6 diphosphate(FDP)(50 mg/kg, n =20), MK 801(1 mg/kg, n =20) and N acetylcystein(NAC)(150 mg/kg, n =20) were singly or combinantly infused intraperitoneally At the same time the cocktail treated group ( n =20) were infused the above agents combinationtly and the control group ( n =20) were infused normal saline intraperitoneally. six and 24 hours after focal cerebral ischemia,the animals were weighted and neurologically assessed on 5 point scale The animals were killed; brain were stained with 2,3,5 triphenylte trazolinm chloride for assessment of the volume of infarction, and then embedden onto slides with paraffin for morphological assessment and terminal transferase dUTP nick ending labeling(TUNEL) were carried out for apoptosis and immunohistochemistry were carried out to investigate the changes in bcl 2 Results All neuroprotectants decreased the volume of infarction( P